365 Turn Off Conversation View in Outlook 365

Look at the top of the e-mail inbox. Find and click the sort header (e.g., Items by Date).
Find the Conversations section of the drop-down menu.
Choose Off.
Note: If you would like to turn it back on, simply repeat the process and choose On.

Mark New Messages as Read Automatically (System Settings)

Click the gear symbol at the top-right of the screen.
Select Options and Outlook will default to the Account options.
Click Settings on the left-side navigation.
Find the Reading Pane section on the right side of the screen.
Find the statement labeled Choose When Items Should Be Marked as Read.
Click the first item, Mark the Item Displayed in the Reading Pane as Read, if you would like to have Outlook change the read/unread status while you are reading any given message. Note: This will trigger read responses that have been requested so people who track such things do not think you have deleted the mail without reading.
Change the number in the box to 1 (second).
Click the Save button at the bottom of the screen.

Click the gear symbol at the top-right of the screen.
Select Options and Outlook will default to the Account options.
Click Settings on the left-side navigation.
Find the E-Mail Signature section at the top of the screen and type the footer that you would like to appear in your mail.
Check the box labeled Automatically include my signature on messages I send if you would like the signature used regularly.
Click the save button at the bottom of the screen.

Click the picture of the silhouette/avatar at the top-right of the screen.
Find and click the Change link immediately beneath the silhouette/avatar.
Click the picture of the file folder.
Navigate to the location of your saved graphic.
Double-click the file. You should briefly see little dots rotating in a circular pattern but nothing else will happen.
Click the Save link.

Click the gear symbol at the top-right of the screen.
Choose Display Settings. The Message List options will load by default.
Click Reading Pane.
Select Show Reading Pane at Bottom.
Check the Apply to All Folders box if you would like this setup for all mail folders.
Click the OK button above the options.

Click the gear symbol at the top-right of the screen.
Choose Set Automatic Replies (also located in Options | Organize E-Mail | Automatic Replies).
Select Send Automatic Replies, identify a timeframe and type the message you would like to use in your reply.
Click the Save button at the bottom of the screen. Note: You may need to scroll down to see it.